Thanks to all for your comments. As with all good fault finding, I'll go through each suggestion.
QUOTE(spinner @ Feb 5 2008, 08:42 AM)

It is possible that both tracks did not rip correctly to the hard drive.
Both tracks have been played before on several occasions without problem, so I would assume that they have been ripped correctly.
QUOTE(Gary @ Feb 5 2008, 01:14 PM)

If you use an external USB hub to run the external drive and say...a USB keyboard (for even faster track searching), check that the power supply for the USB hub hadn't worked loose from the hub (making it an unpowered hub).
I don't have a powered hub, but I've managed over 50 discos without a problem and wasn't doing anything different when it happened.
QUOTE(Gary @ Feb 5 2008, 01:14 PM)

Also, check that the external hard disk drive power supply hadn't worked loose from the back of the external hard drive.
It was plugged in at both ends and the LED was lit and flashing
QUOTE(Gary @ Feb 5 2008, 01:14 PM)

If external hard drive temperature is greater than xx'degrees
The room wasn't any warmer than other venues I've played at and the drive sits on the table so has full ventilation.
With regard to defragging, I would tend to prefer the option of reformatting the drive and then reinstalling, as all the files on the drive are stored elsewhere.
Any other comments appreciated as I'll wait a couple of days before doing anything